Vasopressin/Oxytocin V2R fluorescent probe
Tag-lite Vasopressin/Oxytocin V2 receptor fluorescent probe
Vasopressin V2R stably expressing cells HTRF®
This cell line stably expresses the Vasopressin V2 receptor fused to a SNAP-Tag, and can be used in a Tag-lite application.
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Fully functional cell line
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Stable expression
Overview
There are three subtypes of vasopressin receptor: V1A, V1B, and V2. The V2 receptor is mainly found in the kidney and is important in free water reabsorption.
This HEK293 stable cell line expresses the V2 receptor fused to a SNAP-Tag. Labeled with Terbium, the cells can be used in a Tag-lite receptor binding protocol.
Benefits
- STABLY TRANSFECTED
- HIGH PERCENTAGE OF VIABILITY
- FULLY FUNCTIONAL
Step 1 - Receptor labeling
SNAP-tag® is a small fusion tag that covalently interacts with specific substrates. It enables specific covalent labeling of any protein of interest. For more details, see the labeling procedure.
Watch this video describing how to label cell surface receptors using Tag-lite® technology.
Step 2 - Understand the assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Step 3 - Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Watch this video explaining how to run a saturation binding assay using Tag-lite.
Step 4 - Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Watch this video explaining how to run a competitive binding assay using Tag-lite.
Kd and Ki validation
Examples of data obtained using vasopressin V2-R expressing stable cells (HEK) labelled with terbium in Tag-lite buffer (LABMED), and their matching fluorescent ligand (L0063RED). Vasopressin was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Scientific Presentations
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Scientific Presentations
In collaboration with CNRS - Scientific Presentations
The easy way to approach biology without radioactivity - Flyers
Challenge the limits of binding kinetics studies - Application Notes
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Videos
Product Insert V2-R CL (HEK) / C1PU1V2
C1PU1V2 - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics
Available On-demand - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
A comprehensive overview of pharmacology's main ligands - Guides
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Safety Data Sheet V2-R CL (HEK) / C1PU1V2
C1PU1V2 - Safety Data Sheet
Safety Data Sheet (DEU) V2-R CL (HEK) / C1PU1V2
C1PU1V2 - Safety Data Sheet
Safety Data Sheet (ELL) V2-R CL (HEK) / C1PU1V2
C1PU1V2 - Safety Data Sheet
Safety Data Sheet (FRA-FR) V2-R CL (HEK) / C1PU1V2
C1PU1V2 - Safety Data Sheet
Safety Data Sheet (ITA) V2-R CL (HEK) / C1PU1V2
C1PU1V2 - Safety Data Sheet
Safety Data Sheet (SPA) V2-R CL (HEK) / C1PU1V2
C1PU1V2 - Safety Data Sheet
Safety Data Sheet (ENG-GB) V2-R CL (HEK) / C1PU1V2
C1PU1V2 - Safety Data Sheet
Safety Data Sheet (ENG-US) V2-R CL (HEK) / C1PU1V2
C1PU1V2 - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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