Phospho-AKT1 (Ser473) cellular kit
Simple, all-in-one kit for robust detection of Phoshpo-ATK & Total ATK
PAb Antiphosphothreonine-Eu cryptate HTRF®
PAb Antiphosthreonine-Eu cryptate enables the rapid detection of phosphorylation of peptides or proteins on Thr in a biochemical kinase assay format.
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No-wash
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Ease-of-use
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High affinity
Overview
The Anti-phospho threonine-Eu cryptate is a labeled polyclonal antibody. It is highly specific for the presence of phosphothreonine in peptides or proteins and shows no cross-reactivity with the corresponding non-phosphorylated threonine residues. This Anti-Phosphothreonine antibody does not react with phosphotyrosine or phosphoserine. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.
Benefits
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-(THR) SPECIFIC
Assay principle
HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Antiphosphothreonine-Eu cryptate prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Experience the freedom of building your own assay
HTRF kinase activity toolbox reagents - Flyers
Product Insert A-P threonine-Eu / 61PTRKAE-61PTRKAZ-61PTRKAY
61PTRKAE-61PTRKAZ-61PTRKAY - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Revolutionize your kinases investigations with HTRF
Watch this video to learn more about kinases investigations - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Safety Data Sheet A-P threonine-Eu / 61PTRKAE
61PTRKAE - Safety Data Sheet
Safety Data Sheet (DEU) A-P threonine-Eu / 61PTRKAE
61PTRKAE - Safety Data Sheet
Safety Data Sheet (ELL) A-P threonine-Eu / 61PTRKAE
61PTRKAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P threonine-Eu / 61PTRKAE
61PTRKAE - Safety Data Sheet
Safety Data Sheet (ITA) A-P threonine-Eu / 61PTRKAE
61PTRKAE - Safety Data Sheet
Safety Data Sheet (SPA) A-P threonine-Eu / 61PTRKAE
61PTRKAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P threonine-Eu / 61PTRKAE
61PTRKAE - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P threonine-Eu / 61PTRKAE
61PTRKAE - Safety Data Sheet
Batch Information A-P threonine-Eu / 20230608
61PTRKAE Batch 14A - Batch Information
Batch Information A-P threonine-Eu / 20230723
61PTRKAE Batch 11G - Batch Information
Batch Information A-P threonine-Eu / 20230723
61PTRKAE Batch 11H - Batch Information
Safety Data Sheet A-P threonine-Eu / 61PTRKAY
61PTRKAY - Safety Data Sheet
Safety Data Sheet (DEU) A-P threonine-Eu / 61PTRKAY
61PTRKAY - Safety Data Sheet
Safety Data Sheet (ELL) A-P threonine-Eu / 61PTRKAY
61PTRKAY - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P threonine-Eu / 61PTRKAY
61PTRKAY - Safety Data Sheet
Safety Data Sheet (ITA) A-P threonine-Eu / 61PTRKAY
61PTRKAY - Safety Data Sheet
Safety Data Sheet (SPA) A-P threonine-Eu / 61PTRKAY
61PTRKAY - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P threonine-Eu / 61PTRKAY
61PTRKAY - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P threonine-Eu / 61PTRKAY
61PTRKAY - Safety Data Sheet
Batch Information A-P threonine-Eu / 20230723
61PTRKAY Batch 11 - Batch Information
Safety Data Sheet A-P threonine-Eu / 61PTRKAZ
61PTRKAZ - Safety Data Sheet
Safety Data Sheet (DEU) A-P threonine-Eu / 61PTRKAZ
61PTRKAZ - Safety Data Sheet
Safety Data Sheet (ELL) A-P threonine-Eu / 61PTRKAZ
61PTRKAZ - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P threonine-Eu / 61PTRKAZ
61PTRKAZ - Safety Data Sheet
Safety Data Sheet (ITA) A-P threonine-Eu / 61PTRKAZ
61PTRKAZ - Safety Data Sheet
Safety Data Sheet (SPA) A-P threonine-Eu / 61PTRKAZ
61PTRKAZ - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P threonine-Eu / 61PTRKAZ
61PTRKAZ - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P threonine-Eu / 61PTRKAZ
61PTRKAZ - Safety Data Sheet
Batch Information A-P threonine-Eu / 20230723
61PTRKAZ Batch 11 - Batch Information
Batch Information A-P threonine-Eu / 20230608
61PTRKAZ Batch 14 - Batch Information
Plate Reader Requirement
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