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PAb Anti-phospho PKC substrate-Eu cryptate HTRF®

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  • Discover HTRF kinase solutions
PAb Anti-phospho PKC substrate-Eu cryptate enables the rapid detection of phosphorylation of peptides or proteins on Ser in a biochemical kinase assay format.
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  • No-wash No-wash
  • Ease-of-use Ease-of-use
  • High affinity High affinity
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PAb Anti-phospho PKC substrate-Eu cryptate enables the rapid detection of phosphorylation of peptides or proteins on Ser in a biochemical kinase assay format.
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Overview

The Anti-phospho PKA-Eu cryptate is a labelled polyclonal antibody. It recognizes phosphorylated serine with arginine or lysine residues in the -2 and +2 positions and a hydrophobic residue in the +1 position. It does not cross-react with non-phosphorylated serine residue or phosphorylated serine in a different configuration, nor does it recognize phospho threonine. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.

Benefits

  • NO LIMITATION IN ATP CONCENTRATIONS
  • ANY KIND OF SUBSTRATES
  • PHOSHO-(SER) SPECIFIC

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
HTRF kinase assay principle

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho PKC substrate-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
HTRF kinase assay protocol
View product citations for Kinases 61P03KAE on CiteAb

Open R&D: Sanofi Access Platform

In collaboration with Sanofi - Scientific Presentations

Experience the freedom of building your own assay

HTRF kinase activity toolbox reagents - Flyers

Product Insert A-P PKC-Eu / 61P03KAE-61P03KAZ-61P03KAY

61P03KAE-61P03KAZ-61P03KAY - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

The essential white paper to expand your knowledge on kinases

Learn about cutting edge kinase research in this free white paper - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Revolutionize your kinases investigations with HTRF

Watch this video to learn more about kinases investigations - Videos

Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

HTRF solutions for Kinases

From biochemical to cell based - Brochures

Safety Data Sheet A-P PKC-Eu / 61P03KAE

61P03KAE - Safety Data Sheet

Batch Information A-P PKC-Eu / 20280731

61P03KAE Batch 07A - Batch Information

Safety Data Sheet (DEU) A-P PKC-Eu / 61P03KAE

61P03KAE - Safety Data Sheet

Safety Data Sheet (ELL) A-P PKC-Eu / 61P03KAE

61P03KAE - Safety Data Sheet

Safety Data Sheet (FRA-FR) A-P PKC-Eu / 61P03KAE

61P03KAE - Safety Data Sheet

Safety Data Sheet (ITA) A-P PKC-Eu / 61P03KAE

61P03KAE - Safety Data Sheet

Safety Data Sheet (SPA) A-P PKC-Eu / 61P03KAE

61P03KAE - Safety Data Sheet

Safety Data Sheet (ENG-GB) A-P PKC-Eu / 61P03KAE

61P03KAE - Safety Data Sheet

Safety Data Sheet (ENG-US) A-P PKC-Eu / 61P03KAE

61P03KAE - Safety Data Sheet

Safety Data Sheet A-P PKC-Eu / 61P03KAY

61P03KAY - Safety Data Sheet

Safety Data Sheet (DEU) A-P PKC-Eu / 61P03KAY

61P03KAY - Safety Data Sheet

Safety Data Sheet (ELL) A-P PKC-Eu / 61P03KAY

61P03KAY - Safety Data Sheet

Safety Data Sheet (FRA-FR) A-P PKC-Eu / 61P03KAY

61P03KAY - Safety Data Sheet

Safety Data Sheet (ITA) A-P PKC-Eu / 61P03KAY

61P03KAY - Safety Data Sheet

Safety Data Sheet (SPA) A-P PKC-Eu / 61P03KAY

61P03KAY - Safety Data Sheet

Safety Data Sheet (ENG-GB) A-P PKC-Eu / 61P03KAY

61P03KAY - Safety Data Sheet

Safety Data Sheet (ENG-US) A-P PKC-Eu / 61P03KAY

61P03KAY - Safety Data Sheet

Safety Data Sheet A-P PKC-Eu / 61P03KAZ

61P03KAZ - Safety Data Sheet

Batch Information A-P PKC-Eu / 20280731

61P03KAZ Batch 07A - Batch Information

Safety Data Sheet (DEU) A-P PKC-Eu / 61P03KAZ

61P03KAZ - Safety Data Sheet

Safety Data Sheet (ELL) A-P PKC-Eu / 61P03KAZ

61P03KAZ - Safety Data Sheet

Safety Data Sheet (FRA-FR) A-P PKC-Eu / 61P03KAZ

61P03KAZ - Safety Data Sheet

Safety Data Sheet (ITA) A-P PKC-Eu / 61P03KAZ

61P03KAZ - Safety Data Sheet

Safety Data Sheet (SPA) A-P PKC-Eu / 61P03KAZ

61P03KAZ - Safety Data Sheet

Safety Data Sheet (ENG-GB) A-P PKC-Eu / 61P03KAZ

61P03KAZ - Safety Data Sheet

Safety Data Sheet (ENG-US) A-P PKC-Eu / 61P03KAZ

61P03KAZ - Safety Data Sheet

Plate Reader Requirement

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