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PAb Anti-phospho AKT substrate-Eu cryptate HTRF®

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  • Photography of PAb Anti-phospho AKT substrate-Eu cryptate vial
  • Photography of PAb Anti-phospho AKT substrate-Eu cryptate vial
  • Discover HTRF kinase solutions
PAb Anti-phospho AKT substrate-Eu cryptate enables the rapid detection of Akt phosphorylation on Ser/Thr in a biochemical kinase assay format.
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  • No-wash No-wash
  • Ease-of-use Ease-of-use
  • High affinity High affinity
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PAb Anti-phospho AKT substrate-Eu cryptate enables the rapid detection of Akt phosphorylation on Ser/Thr in a biochemical kinase assay format.
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Overview

The Anti-phospho AKT substrate-Eu cryptate is a labeled polyclonal antibody enabling the detection of Akt when phosphorylated at Ser or Thr preceded by arginine or lysine in -5 and -3 positions. It does not cross-react with phosphorylated Ser or Thr in different configurations (slight cross reactions with -2 and +2 positions). It does not detect the non-phosphorylated Akt. This allows the construction of a specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.

Benefits

  • NO LIMITATION IN ATP CONCENTRATIONS
  • ANY KIND OF SUBSTRATES
  • PHOSHO-AKT (SER/THR) SPECIFIC

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
HTRF kinase assay principle

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho AKT substrate-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
HTRF kinase assay protocol
View product citations for Kinases 61P02KAE on CiteAb

Open R&D: Sanofi Access Platform

In collaboration with Sanofi - Scientific Presentations

Experience the freedom of building your own assay

HTRF kinase activity toolbox reagents - Flyers

Product Insert Anti-P AKT-Eu / 61P02KAE-61P02KAZ-61P02KAY

61P02KAE-61P02KAZ-61P02KAY - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

The essential white paper to expand your knowledge on kinases

Learn about cutting edge kinase research in this free white paper - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Revolutionize your kinases investigations with HTRF

Watch this video to learn more about kinases investigations - Videos

Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

HTRF solutions for Kinases

From biochemical to cell based - Brochures

Safety Data Sheet Anti-P AKT-Eu / 61P02KAE

61P02KAE - Safety Data Sheet

Safety Data Sheet (DEU) Anti-P AKT-Eu / 61P02KAE

61P02KAE - Safety Data Sheet

Safety Data Sheet (ELL) Anti-P AKT-Eu / 61P02KAE

61P02KAE - Safety Data Sheet

Safety Data Sheet (FRA-FR) Anti-P AKT-Eu / 61P02KAE

61P02KAE - Safety Data Sheet

Safety Data Sheet (ITA) Anti-P AKT-Eu / 61P02KAE

61P02KAE - Safety Data Sheet

Safety Data Sheet (SPA) Anti-P AKT-Eu / 61P02KAE

61P02KAE - Safety Data Sheet

Safety Data Sheet (ENG-GB) Anti-P AKT-Eu / 61P02KAE

61P02KAE - Safety Data Sheet

Safety Data Sheet (ENG-US) Anti-P AKT-Eu / 61P02KAE

61P02KAE - Safety Data Sheet

Batch Information Anti-P AKT-Eu / 20230921

61P02KAE Batch 05B - Batch Information

Safety Data Sheet Anti-P AKT-Eu / 61P02KAY

61P02KAY - Safety Data Sheet

Safety Data Sheet (DEU) Anti-P AKT-Eu / 61P02KAY

61P02KAY - Safety Data Sheet

Safety Data Sheet (ELL) Anti-P AKT-Eu / 61P02KAY

61P02KAY - Safety Data Sheet

Safety Data Sheet (FRA-FR) Anti-P AKT-Eu / 61P02KAY

61P02KAY - Safety Data Sheet

Safety Data Sheet (ITA) Anti-P AKT-Eu / 61P02KAY

61P02KAY - Safety Data Sheet

Safety Data Sheet (SPA) Anti-P AKT-Eu / 61P02KAY

61P02KAY - Safety Data Sheet

Safety Data Sheet (ENG-GB) Anti-P AKT-Eu / 61P02KAY

61P02KAY - Safety Data Sheet

Safety Data Sheet (ENG-US) Anti-P AKT-Eu / 61P02KAY

61P02KAY - Safety Data Sheet

Batch Information Anti-P AKT-Eu / 20230921

61P02KAY Batch 05 - Batch Information

Safety Data Sheet Anti-P AKT-Eu / 61P02KAZ

61P02KAZ - Safety Data Sheet

Safety Data Sheet (DEU) Anti-P AKT-Eu / 61P02KAZ

61P02KAZ - Safety Data Sheet

Safety Data Sheet (ELL) Anti-P AKT-Eu / 61P02KAZ

61P02KAZ - Safety Data Sheet

Safety Data Sheet (FRA-FR) Anti-P AKT-Eu / 61P02KAZ

61P02KAZ - Safety Data Sheet

Safety Data Sheet (ITA) Anti-P AKT-Eu / 61P02KAZ

61P02KAZ - Safety Data Sheet

Safety Data Sheet (SPA) Anti-P AKT-Eu / 61P02KAZ

61P02KAZ - Safety Data Sheet

Safety Data Sheet (ENG-GB) Anti-P AKT-Eu / 61P02KAZ

61P02KAZ - Safety Data Sheet

Safety Data Sheet (ENG-US) Anti-P AKT-Eu / 61P02KAZ

61P02KAZ - Safety Data Sheet

Batch Information Anti-P AKT-Eu / 20230921

61P02KAZ Batch 05 - Batch Information

Plate Reader Requirement

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