SNAP tag OX2 OrexinR cloned plasmid
Tag-lite plasmid cloned with a SNAP tag OX2 Orexin receptor
Orexin OX1 and OX2R fluorescent probe HTRF®
This Orexin receptor red agonist is an OrexinA derivative labeled with a red emitting HTRF fluorescent probe.
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Non-radioactive
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No-wash
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Cell-based
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Equilibrium & Kinetic binding compatible
Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This Orexin receptor red agonist is an OrexinA derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Orexin 1 and Orexin 2 receptor binding assays.
Benefits
- FLUORESCENTLY LABELED LIGAND
- HIGHLY STABLE
- VALIDATED IN BINDING
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Watch this video explaining how to run a saturation binding assay using Tag-lite.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Watch this video explaining how to run a competitive binding assay using Tag-lite.
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Scientific Presentations
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Scientific Presentations
The easy way to approach biology without radioactivity - Flyers
Challenge the limits of binding kinetics studies - Application Notes
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Videos
Product Insert Orexin-R red ago / L0025RED
L0025RED - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics
Available On-demand - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
A comprehensive overview of pharmacology's main ligands - Guides
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Safety Data Sheet Orexin-R red ago / L0025RED
L0025RED - Safety Data Sheet
Safety Data Sheet (DEU) Orexin-R red ago / L0025RED
L0025RED - Safety Data Sheet
Safety Data Sheet (ELL) Orexin-R red ago / L0025RED
L0025RED - Safety Data Sheet
Safety Data Sheet (FRA-FR) Orexin-R red ago / L0025RED
L0025RED - Safety Data Sheet
Safety Data Sheet (ITA) Orexin-R red ago / L0025RED
L0025RED - Safety Data Sheet
Safety Data Sheet (SPA) Orexin-R red ago / L0025RED
L0025RED - Safety Data Sheet
Safety Data Sheet (ENG-GB) Orexin-R red ago / L0025RED
L0025RED - Safety Data Sheet
Safety Data Sheet (ENG-US) Orexin-R red ago / L0025RED
L0025RED - Safety Data Sheet
Batch Information Orexin-R red ago / 20230504
L0025RED Batch 12A - Batch Information
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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