Kinase-GST Binding Discovery Kit
The Kinase GST binding Discovery kit is intended for fluorescent inhibitor selection.
Kinase Binding Buffer HTRF®
The Kinase Binding Buffer is a spare part intended for use in the HTRF® Kinase Binding platform only.
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Developed and tested to Cisbio's high quality standards
Overview
The Kinase Binding Buffer is used to perform saturation binding (Kd-determination) and/or competitive binding assays within the HTRF® Kinase Binding platform only.
Benefits
- PRODUCED BY MANUFACTURER
- STRINGENT QUALITY CONTROL
Kd determination assay principal
The binding of a fluorescent tracer is detected in a sandwich assay format using either a specific Anti-GST, 6HIS antibody or Streptavidin labeled with Europium Cryptate (donor), which bind to the tagged Kinase, and a red fluorescent tracer labelled with d2 (acceptor). The detection principle is based on HTRF® technology. The HTRF ratio (665/620) will increase upon the addition of more tracer, and will saturate depending on the dissociation constant (Kd ) of the tracer to the tagged kinase. When an inhibitor of the kinase is added, the tracer will be displaced and the HTRF signal will disappear, depending on the dose.
IC50/Ki determination assay protocol
Pharmacological evaluation of inhibitors of interest can be run in 96- or 384-well plates.
First, a dilution series of inhibitor ranging between 40 µM and 0.23 nM is prepared, and 5 µL of each concentration are dispensed into the plate. Next, 5 µL of tagged-Kinase are added, followed by 5 µL of anti-tag Eu-cryptate. Finally, 5 µL of tracer solution are added, prepared at 4x the final concentration. The HTRF ratio is measured after 1 H of incubation.
Analyses of the data give typical dose response curves ranging between 10 µM and 56 pM, enabling an evaluation of the IC50/Ki values for the inhibitor of interest.
The ultimate guide to successful kinase binding experiments
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3 app notes on kinase inhibitor selection for kinase binding assays
Determine the best tracer to your kinase binding assay - Application Notes
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
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Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
How to run a Kinase Binding Platform assay
What to expect at the bench - Videos
Revolutionize your kinases investigations with HTRF
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Easy method for kinetic binding determination
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Improve PROTAC Targeting Kinase Discovery
Application note describing how HTRF binding kits can help assessing PROTAC targeting kinase - Application Notes
Safety Data Sheet (DEU) Kin bind Buffers / 62KBBRDD
62KBBRDD - Safety Data Sheet
Safety Data Sheet (ELL) Kin bind Buffers / 62KBBRDD
62KBBRDD - Safety Data Sheet
Safety Data Sheet (FRA-FR) Kin bind Buffers / 62KBBRDD
62KBBRDD - Safety Data Sheet
Safety Data Sheet (ITA) Kin bind Buffers / 62KBBRDD
62KBBRDD - Safety Data Sheet
Safety Data Sheet (SPA) Kin bind Buffers / 62KBBRDD
62KBBRDD - Safety Data Sheet
Safety Data Sheet (ENG-GB) Kin bind Buffers / 62KBBRDD
62KBBRDD - Safety Data Sheet
Safety Data Sheet (ENG-US) Kin bind Buffers / 62KBBRDD
62KBBRDD - Safety Data Sheet
Batch Information Kin bind Buffers / 20240117
62KBBRDD Batch 01A - Batch Information
Batch Information Kin bind Buffers / 20260222
62KBBRDD Batch 03A - Batch Information
Batch Information Kin bind Buffers / 20270404
62KBBRDD Batch 04A - Batch Information
Safety Data Sheet (DEU) Kin bind Buffers / 62KBBRDF
62KBBRDF - Safety Data Sheet
Safety Data Sheet (ELL) Kin bind Buffers / 62KBBRDF
62KBBRDF - Safety Data Sheet
Safety Data Sheet (FRA-FR) Kin bind Buffers / 62KBBRDF
62KBBRDF - Safety Data Sheet
Safety Data Sheet (ITA) Kin bind Buffers / 62KBBRDF
62KBBRDF - Safety Data Sheet
Safety Data Sheet (SPA) Kin bind Buffers / 62KBBRDF
62KBBRDF - Safety Data Sheet
Safety Data Sheet (ENG-GB) Kin bind Buffers / 62KBBRDF
62KBBRDF - Safety Data Sheet
Safety Data Sheet (ENG-US) Kin bind Buffers / 62KBBRDF
62KBBRDF - Safety Data Sheet
Batch Information Kin bind Buffers / 20240117
62KBBRDF Batch 01A - Batch Information
Batch Information Kin bind Buffers / 20260222
62KBBRDF Batch 03A - Batch Information
Plate Reader Requirement
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