cAMP Gs dynamic kit
Optimal performance when applied to Gs activation or inhibition
IP-One Gq kit HTRF®
This HTRF kit detects the accumulation of inositol monophosphate, a metabolite produced following phospholipase C activation and used as a proxy of Gq activation
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Ease-of-use
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Low false negative
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Highly specific
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Accurate pharmacology
Overview
Cisbio IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows direct characterization of all types of compounds acting on Gq-coupled receptors in either adherent or suspension cells.
From high-throughput screening (HTS) to structure-activity relationships (SAR), IP-One has been shown to offer superior benefits over traditional assays
Benefits
- SPECIFICALLY TIED TO GQ SIGNALING
- LOW FALSE NEGATIVES
- ACCURATE PHARMACOLOGICAL PROFILES
Assay principle
The kit is based on a competitive format involving a specific antibody labeled with cryptate (donor) and IP1 coupled to d2 (acceptor). Native IP1 produced by cells competes with d2-labeled IP1 for binding to monoclonal anti-IP1 Tb2+ cryptate. LiCl is added to the cell stimulation buffer, causing IP1 to accumulate upon receptor activation.
Assay protocol
The IP-One assay features a streamlined protocol with just two incubation steps: Cell stimulation by the ligand or target compounds IP1 detection using HTRF reagentsThis protocol requires only a single, one-hour incubation period following cell stimulation.
Specificity of IP-One Gq assay
IP-One ensures signal specificity, measuring only IP-1, an analyte that is solely produced by the action of the phospholipase enzyme (PLC) and mediated by GPCRs.
In contrast, calcium responses are complex in nature. Calcium release is frequently triggered by non-G protein mechanisms, including Ca2+ permeable channels, calcium pumps, calcium transporters.
Standard curve characteristics
Assay Window, sensitivity, reproducibility and specificity : IP-One makes no concession !
Experience on HTRF technology in public-private collaborations
Neogenius Pharma - Scientific Presentations
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Scientific Presentations
In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations
In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations
In collaboration with MRC technology - Scientific Presentations
HTRF a versatil approach for 7TM drug discovery
In collaboration with MRC - Scientific Presentations
In collaboration with Roche - Scientific Presentations
In collaboration with Novartis - Scientific Presentations
Case Study: HTS of GPR-54 with IP-One Assay
In collaboration with Harvard Center for Neurodegeneration and Repair - Scientific Presentations
cAMP Fresh and Frozen Cell Usage
In collaboration with Bristol Myers Squibb - Scientific Presentations
IP-One & cAMP HTRF Functional Cell-based Screening
In collaboration with Schering AG - Scientific Presentations
In collaboration with Novartis - Scientific Presentations
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF solutions for NASH - Flyers
HTRF products and supporting scientific content to investifgate GPCRs - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
Characterisation of Inositol Phosphate & cAMP Signalling from GPCRs using HTRF
In collaboration with College of Science, King Saud University, University of Western Australia - Posters
HTRF® technology on the SpectraMax® i3 Multi-Mode Platform
In collaboration with Mollecular Devices - Posters
Multidimentional GPCR investigations - Posters
In collaboration with CEREP - Posters
Differentiation of partial and full GPR40 agonists for the treatment of type 2diabetes
In collaboration with Jansen research & development - Posters
In collaboration with Jansen research & development - Posters
Ultimate guides to master Gq assays
Optimizing IP-One assays has never been easier - Guides
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Guidelines and recommendations for analyzing GPCR 2nd messenger data
Written by a panel of experts - Guides
Measuring Gq activation on brain tissues - Posters
Unique receptor dissociation kinetics of the novel endothelin receptor antagonist macitentan
Characterizing a drug candidate for Gq coupled GPCR - Posters
How to monitor Gq coupled GPCR activation in cells with IP-One
What to expect at the bench - Videos
IP-One, a new dimension in Gq signaling
Video guide - Videos
HTRF IP-One assay: a robust and stable platform for high throughput screening of Gaq-coupled GPCRs
Overcome calcium assay signal's transient nature in Gq studies - Technical Notes
Product Insert IP-One Gq / 62IPAPEB-62IPAPEC-62IPAPEJ
62IPAPEB-62IPAPEC-62IPAPEJ - Product Insert
Immuno-Oncology Drug Discovery
Explore our solutions for Immuno-oncology research - Flyers
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
GPCR in Cardiovascular Research: The Relevance of Camp and IP-1 Second Messenger Assays
See how peer researchers monitor GPCR signaling with HTRF - Application Notes
A comprehensive overview of pharmacology's main ligands - Guides
Fibrosis study investigating the role of GPCR signaling in fibrosis development - Application Notes
GTP binding assay : Gαi agonist and antagonist guides
Discover how to get the most out of your Gαi agonist and antagonist research with our HTRF GTP binding assays. - Guides
GPCR compounds identification and pharmacological characterization with GTP Gi binding assay
Characterization of candidate molecules through GTP binding assays - Application Notes
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Safety Data Sheet IP-One Gq / 62IPAPEB
62IPAPEB - Safety Data Sheet
Safety Data Sheet (DEU) IP-One Gq / 62IPAPEB
62IPAPEB - Safety Data Sheet
Safety Data Sheet (ELL) IP-One Gq / 62IPAPEB
62IPAPEB - Safety Data Sheet
Safety Data Sheet (FRA-FR) IP-One Gq / 62IPAPEB
62IPAPEB - Safety Data Sheet
Safety Data Sheet (ITA) IP-One Gq / 62IPAPEB
62IPAPEB - Safety Data Sheet
Safety Data Sheet (SPA) IP-One Gq / 62IPAPEB
62IPAPEB - Safety Data Sheet
Safety Data Sheet (ENG-GB) IP-One Gq / 62IPAPEB
62IPAPEB - Safety Data Sheet
Safety Data Sheet (ENG-US) IP-One Gq / 62IPAPEB
62IPAPEB - Safety Data Sheet
Safety Data Sheet IP-One Gq / 62IPAPEC
62IPAPEC - Safety Data Sheet
Safety Data Sheet (DEU) IP-One Gq / 62IPAPEC
62IPAPEC - Safety Data Sheet
Safety Data Sheet (ELL) IP-One Gq / 62IPAPEC
62IPAPEC - Safety Data Sheet
Safety Data Sheet (FRA-FR) IP-One Gq / 62IPAPEC
62IPAPEC - Safety Data Sheet
Safety Data Sheet (ITA) IP-One Gq / 62IPAPEC
62IPAPEC - Safety Data Sheet
Safety Data Sheet (SPA) IP-One Gq / 62IPAPEC
62IPAPEC - Safety Data Sheet
Safety Data Sheet (ENG-GB) IP-One Gq / 62IPAPEC
62IPAPEC - Safety Data Sheet
Safety Data Sheet (ENG-US) IP-One Gq / 62IPAPEC
62IPAPEC - Safety Data Sheet
Plate Reader Requirement
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