Human Pro-collagen type 1 standard
Hyaluronic Acid kit HTRF®
The Hyaluronic Acid kit is designed for the rapid detection of HA in cell supernatants.
- Highly specific
- Low sample consumption
Hyaluronic Acid (HA), also called hyaluronan, is an important component of the extracellular matrix contributing significantly to cell proliferation and migration. HA may also be involved in the progression of some malignant tumors. HA acts as a promoter of early inflammation, which is crucial to the whole skin wound-healing process. The Hyaluronic Acid kit is designed for a rapid detection of HA in cell supernatants.
- STUDY OF LIVER FIBROSIS
- INFLAMMATION RESEARCH
Hyaluronic Acid is measured using a sandwich immunoassay involving Hyaluronic Acid Binding Protein (HABP) labelled with terbium Cryptate (donor) and HABP labelled with d2 (acceptor). The intensity of the signal is proportional to the concentration of Hyaluronic Acid present in the sample.
Cells are plated and stimulated in the same 96-well culture plate. Cell supernatants are then transferred into the assay plate for the detection of Hyaluronic Acid by HTRF reagents. This protocol enables the cells' viability and confluence to be monitored. The binding buffer is required to enhance the detection of Hyaluronic Acid on samples. The HABP labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Get the brochure about technology comparison. - Brochures
A comprehensive overview of fibrosis development - Guides
63ADK089PEG-63ADK089PEH - Product Insert
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.Let's find your reader
Phospho-SMAD3 (Ser423/425) cellular kit
All-in-one kit for robust detection of Phospho-SMAD3 (Ser423/425)