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EPIgeneous Binding Domain Discovery kit HTRF®

Associated products
  • EPIgeneous Binding Domain Discovery kit components
  • Binding domain interactions validated for EPIgeneous assays
  • EPIgeneous Binding Domain Discovery kit components
  • Binding domain interactions validated for EPIgeneous assays
The Discovery assay enables to determine which of the EPIgeneous binding domain kits must be used for a given epîgenetic binding domain-histone interaction.
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  • Enables fast assay qualification Enables fast assay qualification
Download the protocols
The Discovery assay enables to determine which of the EPIgeneous binding domain kits must be used for a given epîgenetic binding domain-histone interaction.
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Overview

The EPIgeneous Binding Domain kit series provides a simple biochemical approach to study epigenetic reader interactions with modified histones. The three kits, A, B and C, have been validated on 28 different bromo-, chromo- and tudordomains. All kits are based on a GST-tagged binding domain /biotin-coupled Histone peptide format, and can be run using the same add-and-read single plate protocol. Whenever a given domain is not part of the list of validated interactions, the Discovery Binding Domain kit enables researchers to profile which of the A, B or C kits is the best assay solution.

Benefits

  • GOOD DMSO TOLERANCE
  • HIGH SENSITIVITY
  • LOW PROTEIN OR PEPTIDE CONSUMPTION

Assay principle

In EPIgeneous™ Binding Domain kits, the GST-tagged reader domain protein binding to the biotinylated peptide substrate is detected by a conjugate mix: anti-GST Eu or Tb cryptate-labeled antibody conjugate (donor), and streptavidin conjugated to either d2 or XL665 (acceptor). The interaction of the reader domain with the substrate brings the donor and acceptor dyes into close proximity, and allows FRET to occur upon light excitation. The specific signal at 665 nm is inhibited when a specific compound prevents the reader domain protein from binding to its substrate.
EPIgeneous™ Binding Domain Discovery kit principle

Validated binding domains

Three kits (A, B and C) have already been validated and fully optimized on a selection of 28 key binding domains. For non-validated reader domains, a fourth one, the Discovery Kit, enables researchers to profile which of the A, B or C kits is the best assay solution.
EPIgeneous validated binding domains
BINDING DOMAIN KIT A
BINDING DOMAIN KIT BBINDING DOMAIN KIT C
BRD2(1)
CECR2BRD4(1/2)
BRD3(1)FALZ (BPTF)ATAD2A
BRD4(1)BRD2(2)ATAD2B
CBX 1BRD2(1/2)BRD9

BRD3(2)SMARCA4 (BRG1)

BRD3(1/2)BAZ2B

BRD4(2)

BRDT(1)

BRDT(1/2)

CREBBP

BRD1

BRPF3

TAF1L(2)

TAF1L(1/2)

TAF1(2)

TAF1(1/2)

L3MBTL1

UHRF1
View product citations for Epigenetics 62BDDPEG on CiteAb

EPIgeneous binding domain assays

Robust assay tools to decipher key epigenetic interactions - Flyers

Enabling epigenetics studies from HTS to SAR: a novel HTRF® platform to identify and characterize reader domain inhibitors

Case study: BRD4 bromodomain - Posters

How do HTRF® epigenetic binding domain assays perform compared to other technologies?

BRD4 assay - Posters

Product Insert BromoD Discovery Kit / 62BDDPEG

62BDDPEG - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Safety Data Sheet BromoD Discovery Kit / 62BDDPEG

62BDDPEG - Safety Data Sheet

Safety Data Sheet (DEU) BromoD Discovery Kit / 62BDDPEG

62BDDPEG - Safety Data Sheet

Safety Data Sheet (ELL) BromoD Discovery Kit / 62BDDPEG

62BDDPEG - Safety Data Sheet

Safety Data Sheet (FRA-FR) BromoD Discovery Kit / 62BDDPEG

62BDDPEG - Safety Data Sheet

Safety Data Sheet (ITA) BromoD Discovery Kit / 62BDDPEG

62BDDPEG - Safety Data Sheet

Safety Data Sheet (SPA) BromoD Discovery Kit / 62BDDPEG

62BDDPEG - Safety Data Sheet

Safety Data Sheet (ENG-GB) BromoD Discovery Kit / 62BDDPEG

62BDDPEG - Safety Data Sheet

Safety Data Sheet (ENG-US) BromoD Discovery Kit / 62BDDPEG

62BDDPEG - Safety Data Sheet

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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