Free and total anti-insulin antibody radioimmunoassay kit RIA
Predict the future development of type I diabetes in asymptomatic children, adolescents, and young adults
Differentially diagnose between of type I and type II diabetes Evaluate insulin resistance in patients with established diabetes (type I or type II)
Investigate hypoglycemia in non-diabetic subjects
Detect anti-insulin antibodies induced during a treatment by human or porcine insulin
- ACCURATE AND RELIABLE ASSAY
Anti-insulin antibody (AIAb) detection is based on the demonstration of a specific binding with iodine-125 radiolabeled insulin.
There are two alternative protocols to measure levels of free AIAb or total AIAb.
1- Free anti-insulin antibodies The free AIAb are antibodies not complexed with circulating insulin. They are determined by a radioimmunoprecipitation assay in liquid phase. 50µL of serum containing anti-insulin antibodies are incubated with 125I-radiolabeled insulin. The 125I-insulin bound to the AIAb fraction is separated from the free 125I-insulin fraction by precipitation with PEG. After centrifuging, radioactivity counting of the pellets (bound fraction) enables the calculation of the binding percentage of 125I-insulin.
Assay procedure: 2 h at RT + 10 min at RT + 10 min centrifugation
Working range: negative & positive controls