DELFIA® technology Basics

A proven and robust immunodetection platform​

DELFIA® Assay Principle​

DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of an analyte of interest using lanthanide chelate labeled reagents. Wash steps are used to eliminate unbound reagent.​

DELFIA assays can be performed in either direct antigen, indirect antibody sandwich, or competitive assay formats. The technology can equally be applied to fixed or adherent cells.

DELFIA assay principle

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How DELFIA assays work​

DELFIA has a similar workflow to that of a traditional ELISA. Let’s take the example of an immuno assay setup with a direct antibody coating.​

First the plate is coated with a capture antibody. After an overnight incubation, the plate is washed 3 times. The sample is then added and incubated 1.5 hours before a further 3 washing steps. The detection antibody is incubated for 1.5 hours before 3 more washing steps. At that stage, the analyte is sandwiched between the 2 antibodies. The final addition of an enhancement solution will increase the sensitivity of the assay. The lanthanide chelate is dissociated and a new highly fluorescent chelate is formed within a protective micellar solution. The fluorescence emitted can be measured with a plate reader capable of TRF detection.​

How DELFIA can help you in your research​

While superficially quite similar to an ELISA, DELFIA comes with additional benefits. DELFIA has a stable, time-resolved fluorescent signal which improves the assay dynamic range. DELFIA also offers a multiplexing capability (ability to measure multiple targets within the same sample).​

DELFIA is compatible with complex matrices, such as whole cells, cell membranes, cell lysates, tissue homogenates, serum, plasma, etc. DELFIA covers a wide range of applications from biochemical to cell membrane-based, and cell-based (live or fixed cell) assays.​

Wash-based assay

Wash-based assay

Long signal stability

Long signal stability

Broad sample compatibility

Broad sample compatibility

Wide detectable range

Wide detectable range

Scalable

Scalable

DELFIA expands research possibilities​

Thanks to its unrivalled robustness, biodistribution analyses can be assessed using samples from tested animals (blood, urine, feces, or tissue homogenates), making DELFIA the most universal non-radioactive technological platform.​

From immunoassays to binding and enzymatic studies, to cytotoxicity and cell proliferation assays, DELFIA TRF assays are sure to provide you with unmatched sensitivity!​

Cell proliferation​

Cell proliferation​

Cytotoxicity​

Cytotoxicity​

Binding

Binding

GPCRs

Immuno assay ​

Enzymatic studies

Enzymatic studies​

Greater confidence in your results with powerful microplate readers​


PerkinElmer’s portfolio of plate readers provides the latest detection technologies to meet the diverse assay requirements of labs today. DELFIA assays require a plate reader that is capable of TRF (time-resolved fluorescence) detection. VICTOR® Nivo™, EnSight®, or EnVision® all represent trusted solutions to consider in your investment budget.

VICTOR Nivo microplate reader - PerkinElmer

VICTOR® Nivo™

Industry’s Smallest Multimode Plate Reader

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EnSight microplate reader - PerkinElmer

EnSight®

Unique combination of industry’s leading technologies​

Learn more

EnVision microplate reader - PerkinElmer

EnVision®

Our fastest benchtop multimode plate reader​

Learn more

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